USDOE Human Subjects Research Database, Fiscal Year 1996

Michigan State University

Public Information Contact:

Dr. J.Justin McCormick
B620 West Fee Hall
Michigan State University
East Lansing, MI 48824

Phone: 517-353-7785
Fax: 517-353-9004
Email: mccormi1@cranium.com.msu.edu

Institutional Review Board (IRB):

Projects are approved by an IRB located at: Michigan State University.
The approving IRB operates under a Multiple Project Assurance (MPA) recognized by DOE or by the Department of Health and Human Services (HHS).
MPA number of the IRB: M-1239

Human Subjects Projects:

Number of Human Subjects Projects reported: 1

MSU-93-FG02-87ER60524

Malignant Transformation of Human Fibroblasts by Neutrons and Gamma Radiation: Relationship to Mutations Induced

Project Identifier: MSU-93-FG02-87ER60524

Project Title:

Malignant Transformation of Human Fibroblasts by Neutrons and Gamma Radiation: Relationship to Mutations Induced

Principal Investigator: Dr. J. Justin McCormick

Project started in: 1993
This project ended in Fiscal Year 1996.

Fiscal Year 1996 Funding for Research on Human Subjects:

Project Funding Information:
Project did not receive funding in Fiscal Year 1996.
Project used human subjects in Fiscal Year 1996.

Explanation:

No funds received in FY96.

Funding Sources:

DOE: Office of Health and Environmental Research (OHER)

Amount: $0

Information on Use of Human Subjects:

Project does not involve use of multiple protocols/subprojects.

IRB Review:
Type of Review: Full Board
Most Recent Approval: June 19, 1995
IRB Approval Number: 93-337

Number of Human Subjects who participated in this project/protocol during FY 1996 (10/1/95 - 9/30/96): 2

Type of Human Subjects Involvement:

Collection of Bodily Materials:

Collection of personally identifiable bodily materials (blood or blood products, cells, tissue, organs, waste).

• Use of cells cultured in a laboratory.

(a. Objectives, b. Methodology, c. Ionizing Radiation, Radioactive Substances, or Chemical Substances to which human subjects are exposed, d. Involvement of Human Subjects [d.1. procedures used, d.2. risks if any])

(a) The objective of this research is to determine whether human fibroblasts in culture exhibit a cell cycle sensitivity to transformation by ionizing or neutron radiation. (b) This is carried out by synchronizing a special strain of human fibroblast cells (MSU-1.1) developed in this laboratory and then exposing them to radiation at different points in the cell cycle. The cells are then grown for a few days and then assayed for their ability to form foci. Cells isolated from foci form tumors in athymic mice about 30% of the time. MSU-1.1 cells do not form tumors. Results so far indicate that human fibroblasts are hypersensitive to transformation by ionizing radiation during S phase which is quite different than the hypersensitive phase observed in mouse fibroblasts. They are hypersensitive during G2/M. (c) Since the experiments are carried out on cells in culture, the human who was the donor of the cells used is not exposed to any chemical or radioactive material. (d) This proposal makes use of human fibroblasts in culture. They were originally obtained from foreskin tissue of a newborn with the written permission of the parents. The circumcision was undertaken for medical reasons at the explicit wish of the parents.