USDOE Human Subjects Research Database, Fiscal Year 1996

Columbia University

Public Information Contact:

Dr. Marcelo B. Soares
722 West 168th Street
Unit # 41
New York, NY 10032

Phone: 212-960-2313
Fax: 212-781-3577
Email: cuc@cuccfa.ccc.columbia.edu

Institutional Review Board (IRB):

Projects are approved by an IRB located at: Columbia University.
The approving IRB operates under a Multiple Project Assurance (MPA) recognized by DOE or by the Department of Health and Human Services (HHS).
MPA number of the IRB: M-1376

Human Subjects Projects:

Number of Human Subjects Projects reported: 1

CU-91-2923R

Chromosome-specific cDNAs/STSs

Project Identifier: CU-91-2923R

Project Title:

Chromosome-specific cDNAs/STSs

Principal Investigator: Dr. Marcelo B. Soares

Project started in: 1991

Fiscal Year 1996 Funding for Research on Human Subjects:

Project Funding Information:
Project received funding in Fiscal Year 1996.
Project used human subjects in Fiscal Year 1996.

Funding Sources:

DOE: Office of Health and Environmental Research (OHER)

Amount: $350,000 (Est.)

Information on Use of Human Subjects:

Project does not involve use of multiple protocols/subprojects.

IRB Review:
Type of Review: Expedited
Most Recent Approval: June 25, 1996
IRB Approval Number: 2923R

Number of Human Subjects who participated in this project/protocol during FY 1996 (10/1/95 - 9/30/96): 16

Type of Human Subjects Involvement:

Collection of Bodily Materials:

Collection of personally identifiable bodily materials (blood or blood products, cells, tissue, organs, waste).

• Use of existing bodily materials that were collected for another purpose or project.

(a. Objectives, b. Methodology, c. Ionizing Radiation, Radioactive Substances, or Chemical Substances to which human subjects are exposed, d. Involvement of Human Subjects [d.1. procedures used, d.2. risks if any])

The objective of this project is to construct normalized cDNA libraries from human tissues as a resource to the community to expedite gene identification and mapping. The identities of the human subjects are not known by the PI, thus assuring confidentiality. Total cellular RNA extracted from human tissues (obtained for purposes other than those of this project) is used for construction of cDNA libraries that are directionally cloned in a phagemid vector. cDNA libraries are then normalized according to procedures that we have developed (Soares et al., 1994: PNAS 91,9228-9232; Bonaldo, Lennon and Soares 1996: Genome Research, in press). In addition we are taking advantage of subtractive hybridization strategies to reduce the representation of already identified (arrayed and sequenced) I.M.A.G.E. clones from libraries yet to be surveyed. Moreover, we are attempting to develop methods for construction of full-length cDNA libraries.