USDOE Human Subjects Research Database, fiscal year 1995

Lawrence Livermore National Laboratory

Project Identifier: LLNL-89-104

Project Title:

Characterization of Dentin

Principle Investigator: Dr. John H. Kinney

Project started in: 1989

Fiscal Year 1995 Funding for Research on Human Subjects:

Project Funding Information:
Project received funding in Fiscal Year 1995.
Project used human subjects in Fiscal Year 1995.

Funding Sources:

Non-DOE Federal: National Institute of Dental Research (NIDR)

Amount: $85,000

Information on Use of Human Subjects:

Project does not involve use of multiple protocols/subprojects.

IRB Review:
Type of Review: Full Board
Most Recent Approval: March 22, 1995
IRB Approval Number: 89-104

Number of Human Subjects in the Last Reporting Period for this Project: 62
(Reporting periods vary.)

Type of Human Subjects Involvement:

Collection of Bodily Materials:

Collection of personally identifiable bodily materials (blood or blood products, cells, tissue, organs, waste).

• Use of bodily materials collected from subjects specifically for this project.

Questionnaires, Surveys, Epidemiological Studies:

Use of personally identifiable data from questionnaires, surveys, or epidemiological studies.

• Use of data collected from subjects specifically for this project.

(a. Objectives, b. Methodology, c. Ionizing Radiation, Radioactive Substances, or Chemical Substances to which human subjects are exposed, d. Involvement of Human Subjects [d.1. procedures used, d.2. risks if any])

a) Objectives

This is a research effort aimed at measuring the mechanical properties, chemical and composite structure, and resistance to demineralization in dentin, the mineralized hard tissue beneath the enamel in teeth. Characterization of dentin will lead to improved adhesion and bonding of restorations and improved clinical treatment of caries.

b) Methodology

We have developed several new technologies to explore the mechanical behavior of dentin in its fully hydrated state. Chief among these is the development of a nanoprobe scanning force microscope that allows measurements of hardness and modulus of the constituent materials of dentin with nanometer spatial resolution. In addition, three-dimensional x-ray microscopy/x-ray diffraction is giving us information on how the measured properties are controlled by the structure and chemistry of the dentin, and on how these properties are altered due to age and disease.

c) Ionizing radiation: none

d) Involvement of human subjects

Teeth to be used in this study will be selected from those planned for extraction in the University of California, San Francisco (UCSF) dental and oral surgery clinics. The teeth must be noncarious and unrestored. After collection the teeth will again be screened for potential environmental or dental influences, such as tetracycline treatment during development, which is evident in the enamel. Participation will be voluntary and no specific subject population will be sought. Patients who appear in the oral surgery clinics will be asked if they wish to participate. Patients will be paid $15.00 for participation.

There is no risk to human subjects as a result of this study.