Project Identifier: LLNL-95-101
Project Title:
Sperm Aneuploidy in Men Carrying Mutations in the Cystic Fibrosis Gene and Who Have Congenital Absence of Vas Deferens
Principle Investigator:
Andrew J. Wyrobek
Project started in: 1995
Project Funding Information:
Project received funding in Fiscal Year 1995.
Project used human subjects in Fiscal Year 1995.
Funding Sources:
This is one third of a $210,000 project.
Project does not involve use of multiple protocols/subprojects.
IRB Review:
Type of Review: Full Board
Most Recent Approval: January 18, 1995
IRB Approval Number: 95-101
Number of Human Subjects in the Last Reporting Period for this Project: 4
(Reporting periods vary.)
Type of Human Subjects Involvement:
a. Objectives:
The objective of this study is to determine whether men with cystic fibrosis mutations have more chromosomally abnormal sperm than men who do not have the mutation.
b. Methodology:
A small amount of sperm is smeared onto glass slides and is analyzed for sperm aneuploidy by fluorescence in situ hybridization (FISH) using chromosome specific DNA probes.
c. Ionizing Radiation, Radioactive Substances, or Chemical Substances.
None
d. Involvement of Human Subjects.
1. Men who carry a cystic fibrosis mutation affecting the development of the vas deferens are identified by a physician in a collaborating medical genetics clinic and the physician arranges for a sperm sample. Samples are frozen. All samples are coded to protect the confidentiality of the donors.
2. There is no known risk to semen donors associated with these sperm analyses.