USDOE Human Subjects Research Database, fiscal year 1995

Lawrence Berkeley National Laboratory

Project Identifier: LBNL-90-025-H01

Project Title:

Apolipoprotein-Specific HDL and Cholesterol Transport

Principle Investigator: Dr. Alex V. Nichols

Project started in: 1990

Fiscal Year 1995 Funding for Research on Human Subjects:

Project Funding Information:
Project received funding in Fiscal Year 1995.
Project used human subjects in Fiscal Year 1995.

Funding Sources:

Non-DOE Federal: National Heart, Lung, and Blood Institute (NHLBI)

Amount: $125,000 (Est.)
Comments:

Assignment #HL46281

Information on Use of Human Subjects:

Project does not involve use of multiple protocols/subprojects.

IRB Review:
Type of Review: Full Board
Most Recent Approval: February 17, 1995
IRB Approval Number: 95-2-110

Number of Human Subjects in the Last Reporting Period for this Project: 19
(Reporting periods vary.)

Type of Human Subjects Involvement:

Collection of Bodily Materials:

Collection of personally identifiable bodily materials (blood or blood products, cells, tissue, organs, waste).

• Use of existing bodily materials that were collected for another purpose or project.

(a. Objectives, b. Methodology, c. Ionizing Radiation, Radioactive Substances, or Chemical Substances to which human subjects are exposed, d. Involvement of Human Subjects [d.1. procedures used, d.2. risks if any])

OBJECTIVES

To provide an ultimate understanding of the anti-atherogenic role of plasma HDL (high density lipoprotein) by defining how HDL structure and metabolism affect intravascular and extravascular cholesterol redistribution. This aim is being achieved by the use of model and native lipoprotein particles to define the physical-chemical and metabolic bases for speciation of nascent and plasma apolipoprotein-specific high density lipoproteins; by examination of the remodeling analogs of nascent HDL resulting from their interaction with plasma lipoproteins and how phospholipid species, cholesterol, and lipolysis-derived products may modulate the remodeling process; and by the use of apo-specific analogs to nascent HDL and plasma apo-specific HDL to determine the influence of compositional factors on cholesterol efflux from cells.

METHODOLOGY

Human serum collected under another project (LBNL-79-106-H02) is used as the source for isolation of lipoproteins, proteins and enzymes used in the research described above. In donating blood under the source project, the subject experiences the risks common to clinical venipuncture (possible bruising, remote chance of infection). The Alameda-Contra Costa County blood bank serves as an additional source of blood plasma.