USDOE Human Subjects Research Database, fiscal year 1995

Brookhaven National Laboratory

Project Identifier: BNL-77-146

Project Title:

DNA Repair - Human and E. Coli Photoreactivating Enzymes

Principle Investigator: Dr. Betsy M. Sutherland

Project started in: 1977

Fiscal Year 1995 Funding for Research on Human Subjects:

Project Funding Information:
Project received funding in Fiscal Year 1995.
Project used human subjects in Fiscal Year 1995.

Funding Sources:

DOE: Office of Health and Environmental Research (OHER)

Amount: $10,000 (Est.)

Information on Use of Human Subjects:

Project does not involve use of multiple protocols/subprojects.

IRB Review:
Type of Review: Full Board
Most Recent Approval: March 01, 1995

Number of Human Subjects in the Last Reporting Period for this Project: 1
(Reporting periods vary.)

Type of Human Subjects Involvement:

Collection of Bodily Materials:

Collection of personally identifiable bodily materials (blood or blood products, cells, tissue, organs, waste).

• Use of bodily materials collected from subjects specifically for this project.

(a. Objectives, b. Methodology, c. Ionizing Radiation, Radioactive Substances, or Chemical Substances to which human subjects are exposed, d. Involvement of Human Subjects [d.1. procedures used, d.2. risks if any])

We are studying DNA damage and repair in human tissues. Our recent studies indicate that repair in human tissues is much more rapid than in human cells in culture, and make these studies critical to understanding the ability of people to cope with DNA damaging agents in the environment. We use white blood cells from healthy human volunteers in these experiments; the volunteers donate small quantities by approved medical procedure. After withdrawal of blood from the volunteers, the blood cells are separated and used as a source of photoreactivating enzyme, which repairs cyclobutyl pyrimidine dimers in DNA, as well as a source of non-cultured human cells for studies of DNA damage and repair. For studies to date, all DNA damage has been inflicted by ultraviolet lamps or Cesium source; funds are being sought from National Aeronautics and Space Administration (NASA) to allow study of damage induced by HZE (high atomic number, high energy) particles.