USDOE Human Subjects Research Database, Fiscal Year 2000

Consortium for Risk Evaluation w/ Stakeholder Participation

Public Information Contact:

Dr. Charles W. Powers
Executive Director
Consortium for Risk Evaluation and Stakeholder Participation
317 George Street, Suite 202
New Brunswick, NJ 08901-2008

Phone: 732 235-9600
Fax: 732 235-9607
E-mail: cwpowers@eohsi.rutgers.edu

Institutional Review Board (IRB):

Projects are approved by an IRB located at: Univ. of Wash., Univ. of Med. & Dent of NJ, & Rutgers Univ
The approving IRB operates under a Multiple Project Assurance (MPA) recognized by DOE or by the Department of Health and Human Services (HHS).
MPA number of the IRB: M1183, M1467, M1370

Human Subject Projects:

Number of Human Subjects projects reported: 4

CRESP-95-X-0887
"An Update of The Worker Cohort Mortality Study at Nuclear Fuels Production Facility on the Savannah River site "
CRESP-96-27-0009-E
"Laboratory Evaluation of Field Collected Cells"
CRESP-97-27-0228-C
"Screening for Chronic Beryllium Disease at Hanford "
CRESP-98-29-0140 C/B_V
"Surveillance Methods for Solvent-Related Hepatotoxicity"

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Project Identifier: CRESP-95-X-0887

Project Title:
"An Update of The Worker Cohort Mortality Study at Nuclear Fuels Production Facility on the Savannah River site"

Principal Investigator: Dr. Daniel Wartenberg, Rutgers University

Project started in: 1995


Project Funding Information:

This project received funding during fiscal year 2000.
This project used human subjects in fiscal year 2000.

Funding for Human Subjects Research:

DOE: Environmental Management (EM)
$70,000.00 (Est.) for: Fiscal Year 2000

Information on Use of Human Subjects:

This project does not involve the use of multiple protocols/subprojects.

Identifier or number: X-0887

Institutional Review Board (IRB) Review:
Type of Review: Full Board
Approving Institution: University of Medicine and Dentistry of New Jersey
Most recent approval: 09/27/99
IRB approval number: X-0887
Explanation of IRB approval:
An application is in process for IRB approval through the University of Medicine and Dentistry of New Jersey. Project was reviewed and accepted by SRS IRB chairman James O. Hightower January 6, 2000. Notification was made by letter.

Additional IRB approvals from other institutions:
Type of Review: Expedited
Approving Institution: Savannah River Site
Most recent approval: 01/06/00

Number of human subjects who participated in this project/protocol/subproject in the last reporting period: 16489
Reporting period for number of human subjects: Fiscal Year 2000 (10/1/99-9/30/2000)

Type(s) of Human Subjects Involvement:

Use of personally identifiable data from questionnaires, surveys, or epidemiological studies:
Abstract:
(a. Objectives, b. Methodology, c. Ionizing Radiation, Radioactive Substances, or Chemical Substances to which human subjects are exposed, d. Involvement of Human Subjects [d.1. procedures used, d.2. risks if any])

This Study investigates whether workers at the Savannah Power Site (SRS) are more likely, on average, to die of specific causes of death, particularly certain cancers, than the total US population. In Phase I, we will compare causes of death of SRS workers to the causes of death of the total US population, for each gender male or female and race (white or African American ) group. In Phase II of our study, we will use data based on the records of radiation badges that record the dose of external radiation that each worker was exposed to over the period of time that they worked at SRS to determine whether greater radiation exposure is associated with particular causes of death. In Phase III of our study we will examine the possible risk from radiation due to radionuclide exposure (including tritium).


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Project Identifier: CRESP-96-27-0009-E

Project Title:
"Laboratory Evaluation of Field Collected Cells"

Principal Investigator: Dr. Timothy K. Takaro, University of Washington

Project started in: 1996


Project Funding Information:

This project did not receive funding during fiscal year 2000.
This project did not use human subjects in fiscal year 2000.
Explanation: Due to difficulties in recruiting subjects no additional progress has been made since last year's report (9/98)

Funding for Human Subjects Research: No Funding Sources Reported


Information on Use of Human Subjects:

This project does not involve the use of multiple protocols/subprojects.

Institutional Review Board (IRB) Review:
Type of Review: Full Board
Approving Institution: University of Washington
Most recent approval: 08/26/99
IRB approval number: 96-2971-E04
Explanation of IRB approval:
The project was not active between 10/1/99 and 9/30/00.

Number of human subjects who participated in this project/protocol/subproject in the last reporting period: 0
Reporting period for number of human subjects: Fiscal Year 2000 (10/1/99-9/30/2000)

Type(s) of Human Subjects Involvement:

Collection of personally identifiable bodily materials (blood or blood products, urine, cells, tissue, teeth, organs, excretia, etc):
Abstract:
(a. Objectives, b. Methodology, c. Ionizing Radiation, Radioactive Substances, or Chemical Substances to which human subjects are exposed, d. Involvement of Human Subjects [d.1. procedures used, d.2. risks if any])

Heat Stress is a significant exposure for remediation workers at hazardous waste sites. This study will determine if mRNA heat shock proteins up-regulate in heat stressed Hanford remediation workers, and if this biomarker can be used to assess risk from such exposure.

Uroepitheleal cells are harvested from routine urine collections at the Hanford site, and the mRNA activity is analyzed in the laboratory at the University of Washington. The subjects are workers at the Hanford nuclear Reservations tank farms that were recruited for this study on heat stress. The tank farms at Hanford are located along the Columbia River and often experience summer temperatures above 90 degrees Fahrenheit. Because of the extremely hazardous contents of the tanks and the poor characterization of their contents, fully self-contained occlusive suits are often worn by workers in this area.

Methods

Voided urine samples are collected before and after the work shift. Following the measurement of specific gravity and the pH, the sample is centrifuged, and the cell pellet is collected and resuspended in a solution containing RNAse inhibitors. The resuspended cells are then frozen on dry ice, and are transported to the laboratory. Commercially available cDNA stress response gene probes are used to characterize the induction of the stress response in uroepithelial cells. The probes include genes coding for the heat stress proteins of the Hsc70 family, and more general stress response genes, such as thiol glutathione, Gadd43, Gadd153 and p21(Waf1). Because there is a wide variation in the species and quantity of mRNA induction depending upon the cell type, this study aims to identify the stress genes that are most responsive in the transitional uroepthelieal cells harvested in a routinely voided urine sample.

The only samples obtained from the subjects are routine voided spot urine samples collected in privacy. University of Washington investigators will not know the identity of the subjects at any time. Data are identified by a number with the consent form, which is the only document having both a name and number. A member of a cooperating research group from Michigan State University will maintain the identification information.

Adverse effects are not anticipated as subjects will only provide investigators with a urine specimen.

Due to difficulties in recruiting subjects, no additional progress has been made since last years report(9/98).


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Project Identifier: CRESP-97-27-0228-C

Project Title:
"Screening for Chronic Beryllium Disease at Hanford"

See also: PNNL-97-10_V

Principal Investigator: Dr. Timothy K. Takaro, University of Washington

Project started in: 1997


Project Funding Information:

This project received funding during fiscal year 2000.
This project used human subjects in fiscal year 2000.

Funding for Human Subjects Research:

DOE: Environmental Management (EM)
$12,624.00 (Est.) for: Fiscal Year 2000

Information on Use of Human Subjects:

This project does not involve the use of multiple protocols/subprojects.

Institutional Review Board (IRB) Review:
Type of Review: Full Board
Approving Institution: University of Washington
Most recent approval: 07/07/00
IRB approval number: 97-3907-CE

Number of human subjects who participated in this project/protocol/subproject in the last reporting period: 8
Reporting period for number of human subjects: Fiscal Year 2000 (10/1/99-9/30/2000)

Type(s) of Human Subjects Involvement:

Collection of personally identifiable bodily materials (blood or blood products, urine, cells, tissue, teeth, organs, excretia, etc):
Use of personally identifiable data from questionnaires, surveys, or epidemiological studies:
Use of human beings to develop/test instruments, materials, devices, objects, and the like or to investigate the "man-machine interface".
Abstract:
(a. Objectives, b. Methodology, c. Ionizing Radiation, Radioactive Substances, or Chemical Substances to which human subjects are exposed, d. Involvement of Human Subjects [d.1. procedures used, d.2. risks if any])

Investigators obtain names of potential participants for this study from the DOE. Subjects that respond are requested to provide information about work experience, exposures, smoking, and health status; and provide permission to review employment and medical records to determine the extent of beryllium exposure that they may have encountered.

Workers with prior exposure to beryllium are consented to provide 40ml of peripheral blood. The standard venous blood draw is slightly painful at the moment of venipuncture. Rarely, local transient swelling occurs and there is an extremely rare risk of infection from the procedure. Blood cells are isolated, cultured, and tested for beryllium sensitization using the lymphocyte proliferation test.

Personal identification is kept in a separate location from other information. Subjects sign an informed consent form that has been approved by the human subjects review committee.

In this period, the primary emphasis has been on identifying subjects, characterizing their exposure, and testing for beryllium sensitization among exposed workers. Until now, blood samples from 125 Hanford workers were collected and tested for beryllium sensitization under this protocol. Unidentified blood test results, clinical and exposure data are now also collected. This modification was approved July 14, 1999 by the UW IRB and July 17, 1999 by the Pacific Northwest National Labs (PNNL) IRB. During the report period we also received de-identified data from two separate US-DOE grant projects. Another major point of emphasis has been the development of a quantitative decision analytic framework for the use of biomarkers in medical surveillance.


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Project Identifier: CRESP-98-29-0140 C/B_V

Project Title:
"Surveillance Methods for Solvent-Related Hepatotoxicity"

See project NIOSH-98-032.


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